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The inhibition effect of non-protein thiols on dentinal matrix metalloproteinase activity and HEMA cytotoxicity.
J Dent. 2013 Dec 4;
Authors: Nassar M, Hiraishi N, Shimokawa H, Tamura Y, Otsuki M, Kasugai S, Ohya K, Tagami J
Abstract
OBJECTIVES: Phosphoric acid (PA) etching used in etch-and-rinse adhesives is known to activate host-derived dentinal matrix-metalloproteinases (MMPs) and increase dentinal permeability. These two phenomena will result, respectively; in degradation of dentin-adhesive bond and leaching of some monomers especially 2-hydroxyethyl methacrylate (HEMA) into the pulp that would negatively affect the viability of pulpal cells. This study is the first to investigate the inhibitory effect of non-protein thiols (NPSH); namely reduced glutathione (GSH) and N-acetylcysteine (NAC) on dentinal MMPs and compare their effects on HEMA cytotoxicity.
METHODS: Dentin powder was prepared from human teeth, demineralized with 1% PA and then treated with 2% GSH, 2% NAC or 2% chlorhexidine (CHX). Zymographic analysis of extracted proteins was performed. To evaluate the effect of GSH, NAC and CHX on HEMA cytotoxicity, solutions of these compounds were prepared with or without HEMA and rat pulpal cells were treated with the tested solutions for (6 and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity data were analyzed by one-way ANOVA and Tukey post-hoc tests (P<0.05). Results: The inhibitory effect of GSH and NAC on dentinal MMPs was confirmed. GSH showed similar effectiveness to NAC regarding HEMA cytotoxicity inhibition.
CONCLUSION: NPSH were effective to inhibit dentinal MMPs and HEMA cytotoxicity.
CLINICAL SIGNIFICANCE: The tested properties of NPSH provide promising clinical use of these agents which would enhance dentin-bond durability and decrease post-operative sensitivity.
[cite source='pubmed']24316344[/cite] – as supplied by publisher]