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The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength.
Int Endod J. 2013 Oct 9;
Authors: Nassar M, Hiraishi N, Islam MS, Tamura Y, Otsuki M, Kasugai S, Ohya K, Tagami J, Tay F
Abstract
AIM:
To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive.
METHODOLOGY:
The cytotoxicity of 10 mmol L-1 HEMA, 10 mmol L-1 HEMA + 1 mmol L-1 GSH, 10 mmol L-1 HEMA + 5 mmol L-1 GSH and 10 mmol L-1 HEMA + 10 mmol L-1 GSH was compared (6 h and 24 h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1 day of water-storage at 37 °C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P < 0.05).
RESULTS:
There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10 mmol L-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5 mmol L-1 GSH lost its protective effect at 24-h exposure time and 1 mmol L-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5% GSH had higher bond strength value when compared to 10% GSH (P = 0.003).
CONCLUSION:
Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.
[cite source='pubmed']24117849[/cite] – as supplied by publisher]