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The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength.
Int Endod J. 2013 Oct 9;
Authors: Nassar M, Hiraishi N, Islam, Tamura Y, Otsuki M, Kasugai S, Ohya K, Tagami J, Tay F
Abstract
AIM: To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells, and evaluated the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive.
METHODOLOGY: The cytotoxicity of 10 mM HEMA, 10 mM HEMA+1 mM GSH, 10 mM HEMA+5 mM GSH and 10 mM HEMA+10 mM GSH was compared (6 h and 24 h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1 day of water-storage at 37 °C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analyzed by one-way ANOVA and Tukey post-hoc tests (P < 0.05).
RESULTS: There were significant differences among the groups. HEMA elicited a remarkable toxic effect. 10 mM GSH prevented HEMA-induced damage at both exposure times. While 5 mM GSH lost its protective effect at 24-hour exposure time and 1 mM GSH showed no protective effect at both exposure times. GSH had no significant effect on the immediate μTBS; however, 5% GSH had the higher bond strength value when compared to 10% GSH (P = 0.003).
CONCLUSION: Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS This article is protected by copyright. All rights reserved.
[cite source='pubmed']24117849[/cite] – as supplied by publisher]