J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

J Conserv Dent Endod. 2025 Dec;28(12):1246-1251. doi: 10.4103/JCDE.JCDE_771_25. Epub 2025 Dec 9.

ABSTRACT

AIMS: The aim is to evaluate the effect of a strontium chloride-incorporated hypochlorous acid (Sr/HOCl) conditioner on bond performance in eroded dentine using self-etch adhesives.

CONTEXT: Adhesive bonding to eroded dentine presents significant challenges owing to the compromised substrate ultrastructure, exposed collagen networks, and reduced resin infiltration, necessitating innovative pretreatment approaches.

SUBJECTS AND METHODS: Seventy-four caries-free molars were subjected to a 5-day erosive cycling using Coca-Cola. The specimens were randomly allocated to four groups based on the Sr/HOCl pretreatment and adhesive system: Clearfil SE Bond 2 (SEB) and Clearfil Universal Bond Quick (UBQ). Microtensile bond strength testing, failure mode analysis, scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS), and confocal laser scanning microscopy (CLSM) were conducted. Three-way ANOVA with Tukey post hoc comparisons, Chi-square test for failure modes, and t-test for elemental analysis (α =0.05).

RESULTS: UBQ with Sr/HOCl pretreatment significantly improved bond strength (P < 0.001), whereas SEB showed no significant changes (P > 0.05). Mixed failures were predominant across groups. EDS confirmed exclusive strontium incorporation into the treated specimens (P < 0.001), with preserved calcium and phosphorus levels. CLSM revealed reduced interface permeability and enhanced resin infiltration following Sr/HOCl treatment.

CONCLUSIONS: Sr/HOCl conditioning significantly enhanced the bonding to eroded dentine through organic component reduction, strontium deposition, and improved interface characteristics. This pretreatment particularly benefits simplified adhesive systems, suggesting its clinical potential for improving the durability of eroded substrates.

PMID:41438441 | PMC:PMC12721429 | DOI:10.4103/JCDE.JCDE_771_25

Sci Rep. 2025 Jul 1;15(1):21438. doi: 10.1038/s41598-025-06191-7.

ABSTRACT

To evaluate the effects of hypochlorous acid (HOCl) conditioning on bond strength to caries-affected dentin (CAD), antibacterial efficacy against Streptococcus mutans (S. mutans), and dental pulp cell compatibility. Extracted human molars with occlusal caries were pretreated with 50 ppm HOCl before adhesive application (Clearfil SE Bond 2), with untreated specimens serving as controls. Microtensile bond strength (µTBS) was evaluated after 24-hour storage and 10,000 thermocycles. Antibacterial efficacy was assessed by colony-forming unit (CFU) enumeration following S. mutans incubation with HOCl solution. Rat dental pulp cells were cultured with HOCl at concentrations of 0, 0.5, 1, 5, 10, and 50 ppm. Cell viability was assessed using Cell Counting Kit-8. Data underwent statistical analysis using one-way ANOVA with Tukey’s post hoc test (α = 0.05). HOCl pretreatment significantly enhanced µTBS to CAD (from 25.6 ± 4.3 to 35.8 ± 7.2, p < 0.001) and maintained bond stability after thermocycling (32.1 ± 4.5, p > 0.05). HOCl demonstrated significant antibacterial efficacy against S. mutans with substantial reduction of viable bacteria (p < 0.001). Additionally, HOCl maintained compatibility with dental pulp cells. HOCl shows a promise as a dentin conditioner, offering enhance bond performance to CAD with improved bond durability, while providing antibacterial benefits and maintaining biocompatibility.

PMID:40596309 | DOI:10.1038/s41598-025-06191-7